Tempeh Antioxidant Activity using DPPH Method: Effects of Fermentation, Processing, and Microorganisms. Journal of Food Engineering and Technology 2019;8(2):75-80 https://doi.org/10.32732/jfet.2019.8.2.75

 Journal of Food Engineering and Technology 2019;8(2):75-80

https://doi.org/10.32732/jfet.2019.8.2.75


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trademark of
Tempeh Antioxidant Activity using DPPH Method: Effects of
Fermentation, Processing, and Microorganisms
Tati Barus*, Novalin Novita Titarsole, Noryawati Mulyono, Vivitri Dewi Prasasty
Faculty of Biotechnology, Atma
Jaya Catholic University
of Indonesia, Jakarta, Indonesia
E- mail:
: tati.barus@atmajaya.ac.id
Received:
25 September 2019; Accepted: 20 October 2019; Available online
: 30 November 2019
Abstract:
Tempeh is the main type of traditional Indonesian food that is processed from soybeans fermented by
Rhizopus microsporus
. This study aims to measure the antioxidant activity of some kind tempeh using 2,2
-
diphenyl
-1-
picrylhydrazyl (DPPH). Measurement of ant
ioxidant activity toward tempeh took directly from the
producer, tempeh produced on a laboratory scale using
Rhizopus spp
.,
Bacillus spp
., and
Klebsiella sp
. K110,
tempeh fried and steamed, and tempeh during the fermentation stage. The results showed that the fermentation
process soybeans into soybean increase antioxidant activity. The antioxidant activity of tempeh from producers
varies between approximately 5
2- 70%. It is because of tempeh fermented at uncontrol conditions so that the
microorganisms involved in the fermentation time is also uncontrol. Potential microorganisms vary in determining
antioxidant activity. In the group of
Rhizopus spp.
(ATH 35, ATH 24, ATH 53), it showed that the highest
antioxidant activity was found in ATH 35 (8
4%). In the grou
p of
Bacillus spp
. the highest antioxidant activity
produced by
B. megaterium
(76%) and higher than the
Klebsiella sp.
K110 (75%). Thus, the fermented soybeans
into tempeh increase antioxidant activity. The existence of the antioxidant activity of tempeh w
as affected by
strains of microorganisms involved during fermentation and processing time that will be consumed.
Keywords
: Soybean;
Tempeh
; Microorganisms
; Antioxidant
activity; DPPH.

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